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Researchers at IRIG have developed an innovative method to observe photosynthesis at the scale of a single cell using confocal microscopy. It unveils the specialization of various tissues and organisms, showcasing their photoprotection mechanisms in response to light.
For a long time, photo-autotrophic organisms have captured researchers' attention, as they serve as entry points for carbon in the life cycle and are the primary CO2 fixers in the living world. Researchers at IRIG have developed an innovative approach to photo-physiology at the unicellular and subcellular levels. It is crucial to unravel the mechanisms optimizing photosynthesis at the individual cell level since light undergoes various transformations as it penetrates different cellular layers and even organs. Moreover, within the tissue, cells can react differently depending on their developmental stage or physiology.
Researchers from IRIG, internationally renowned, have enhanced photosynthesis evaluation by combining a confocal microscope with saturating pulses to achieve the following three essential objectives:
This simple method can be adapted to various sample types, serving as a versatile tool for studying plant and microalgae photosynthetic acclimation and, consequently, for CO2 capture.
Fundings: Europenan Research Council, ERC Chloro-mito (grant n°833184) the European Union H2020 Project BIOTEC-02-2019 GAIN4CROPS (grant no 862087)
Storti M, Hsine H, Uwizeye C, Bastien O, Yee D, Chevalier F, Decelle J, Giustini C, Beal D, Curien G, Finazzi G, and Tolleter D.
Tailoring confocal microscopy for real-time analysis of photosynthesis at single-cell resolutionCell Reports Methods 2023
CEA is a French government-funded technological research organisation in four main areas: low-carbon energies, defense and security, information technologies and health technologies. A prominent player in the European Research Area, it is involved in setting up collaborative projects with many partners around the world.