Vous êtes ici : Accueil > L'institut > CryoEM to characterize lipid features in membrane tubules scaffolded by yeast eisosome BAR-domain protein Pil1

Agenda


CryoEM to characterize lipid features in membrane tubules scaffolded by yeast eisosome BAR-domain protein Pil1

Vendredi 02 décembre 2022 à 11:00, Salle de séminaire IBS, 71 avenue des Martyrs, Grenoble

Publié le 2 décembre 2022
Jennifer M. Kefauver
University of Geneva, Geneva, Switzerland
Eisosomes are stable specialized plasma membrane (PM) domains of S. cerevisiae that are enriched in sphingolipids and ergosterol. These furrow-like, elongated structures are formed by a lattice of the highly abundant BAR-domain proteins Pil1 and Lsp1. Eisosomes are proposed to be sensitive to membrane stress (i.e. hypo-osmotic shock, heat shock, and mechanical pressure) and may be functionally analogous to caveoli in vertebrate cells. We have purified membrane-bound Pil1/Lsp1 filaments and using cryo-electron microscopy and helical reconstruction, have solved several structures of these filaments. Due to our gentle purification methods, these filaments likely preserve PM lipids in a native-like arrangement and give us a unique window into the lipid behavior of a specialized PM lipid domain.

L’accès au campus EPN nécessite un avis de rendez-vous.
Merci de contacter Odile Cavoret (au moins 48h à l’avance) au 04 57 42 87 04 / ibs.seminaires@ibs.fr
N’oubliez pas de vous munir d’une pièce d’identité.