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Visualization of Competence Development and Transformation in Streptococcus pneumoniae

Vendredi 09 décembre 2022 à 11:00, Salle de séminaire IBS, 71 avenue des Martyrs, Grenoble

Publié le 9 décembre 2022
Nathalie Campo
Centre de Biologie Intégrative de Toulouse
Natural genetic transformation is a conserved mechanism of horizontal gene transfer enabling bacteria to acquire new traits. It requires the development of competence, a specialized physiological state during which exogenous DNA is imported into the cytoplasm and integrated into the chromosome by homologous recombination. In the human pathogen Streptococcus pneumoniae, competence is induced during exponential growth in response to stresses and propagates through the whole population for a short period of time (~25 min, shorter than the generation time of a single cell). Here, I will present different strategies based on fluorescence microscopy that we have developed to monitor the entire transformation process in live pneumococcal cells at the single cell level.

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